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"ABSTRAKPengetahuan lokal mengenai pemanfaatan tumbuhan pangan oleh masyarakat etnis Karo di Desa Semangat Gunung belum sepenuhnya terdokumentasi. Sementara itu, pengaruh budaya lain yang masuk dapat mengancam keberadaan pengetahuan lokal masyarakat. Pendekatan etnobotani dilakukan untuk mendokumentasikan pengetahuan lokal masyarakat mengenai pemanfaatan tumbuhan pangan. Data diperoleh melalui wawancara semistruktural dan terbuka, observasi partisipasi, dan skoring kepada masyarakat lokal. Analisis data dilakukan dengan pendekatan kualitatif, yaitu mengelompokkan tumbuhan pangan berdasarkan kategori guna dan pendekatan kuantitatif, yaitu analisis LUVI, ICS, dan FL. Hasil penelitian diperoleh 109 spesies tumbuhan pangan dimanfaatkan oleh masyarakat, yang dikelompokkan menjadi pangan utama, pengganti pangan utama, sayuran, buah-buahan, bumbu, pangan adat, kudapan, minuman, dan pembungkus makanan. Nilai LUVI tertinggi berupa pangan utama, nilai ICS tertinggi berupa tualah (Cocos nucifera), dan nilai FL tertinggi diperoleh 92 spesies.

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ABSTRACT

Utilization of food plants by the society of Karo ethnic in Semangat Gunung village have not documented yet. The influence of other ethnic cultures that infiltrate to Semangat Gunung village can threaten the local knowledge of its society. Ethnobotanical approach is used to document the local knowledge of the society about food plants utilization. The data were obtained by open-ended and semistructural interview, participant observation, and scoring. The data analysis were carried out by categorizing food plant species based on their use and quantitatively by measuring LUVI, ICS, and FL. The food plant species that used by the society is 109 species. It categorized into 9 subcategory, they are the staple food, alternative staple foods, vegetables, fruits, spices, indigenous foods, snacks, drinks, and food warps. The highest LUVI is staple food subcategory, the highest ICS is tualah (Cocos nucifera), and the highest FL amounts to 92 species."

"ABSTRAKPengaruh eksudat akar dan ekstrak daun Helianlhus mmuus serta Ipomoea batala' terhadap pertumbuhan rumput gajah Petmiselum po/ystacltyon telah dilakulrnn; diamati pula pertumbuhannya bila ditanam di dalam 1 pot bersama H. annuus atau l batatas.Pada penelitian ini. eksudat akar H. mvruus mampu menurunkan persentase perkecambahan biji dan panjang kecambah rumput gajah sampai 23,87 dan 47 % terhadap kontrol dalam 90 jam; sementara eksudat akar I. halalas menunjukkan penurunan 22,37% dan 23,83 %.Eksudat akar dari tanaman bunga matahari yang disiramkan selama 5 minggu pada rumput gajah umur 10 hari menekan tinggi gulma itu 13,62%; berat segar dan berat kerlng 39,56 dan 51,24 %. Eksudat akar tanarnan ubi jalar hanya berpengaruh menekan berat segardan berat kerlng gulma itu sampai 18,58 dan 18,40%.Ekstrak daun H. anmms sertal. hatatas 4 % b.k. mampu mengbambat persentase perl:ecambahan dan tinggi kecambah rumput gajah dalam 90 jam berturut-turut sebesar 38,45 dan 15,28% serta 30,79 dan 19,45%.Ekstrak daun H. annuus sertal. halalas 2% b.k.yang disiramkan sekali seminggu Rumput gajah yang ditanam dalam 1 pot bersama H. annuus atau l halatas tidak menunjukkan perbedaan nyata dalam tinggi dan berat segar rumput gajah; namun berat kering menunjukkan penurunan 33,44 dan 39,63% terhadap kontrol.

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ABSTRACT

Pennisetum polystachyon is native of Tropical Africa. It has a high reproductive capacity and rapid seed germinationit becomes a troublesome weed when it takes over waste- and cultivated lands. It is now also found along the road sides and highways in Indonesia. The seeds are wind dispersed and have a resilient ability to survive drought and certain cultural and chemical control methods.

The concept that some crop plants may be allelopathic to certain weeds is receiving increased attention in the search for alternative weed control strategies. Helianthus ammus and Ipomoea aquatica are amongst the crop plants that may have the allelopathic effect to some weeds.

This research aims to study the inhibiting potential of H. mmuus and I. batatas on the growth of P. polystachyon. This study observed the effects of root exudates and leaf extrects of H. annuus and /. batatas on the germination and growth of P. polystachyon; and also on the growth of this weed grown together with H. a1muus or I. batatas.

Root exudates taken from the sand planted with H. mmuus or I. halalaS for 4 Root exudates liquid comes out from watering H. ammus or/. batatas (01, 2, 3 and 4 plants/pot), poured over a 10-days seedling of P. polyslachyon twice a day during 5 weeks (5 replications each) reduced the height, fresh and dry weight of the weed. The height reduced up to 13.62 %; fresh and dry weight up to 39.56% and 51.24% by the root exudate of H. annuus; while those of L batatas had no effect to the height of P. polystachyon hut did reduce the fresh and dry weight up to18.58 and 18.40%.

Leaf extracts of H. anmms or I. halalas (0--4 % dry weight), with 5 replications each, reduced also the percentage of germination and length of the germination of seed ofP. polystachyon. During 90 hours, leaf extracts of H. ammus reduced the percentage of gennination of the weed species up to 38.45 % and length of the germination of seeds up to 15.28 %; while those of L halalas up to 30.79% and 19.45% respectively.

The I0 days-old seedling of P. polystachyon with SO mlleaf extracts of H. anmtus or L halalas (0; 0.5; J.O; 1.5; and 2% dry weight) once a week, showed a little difference effect on those weed growth a week after the third treatment. Leaf extract of H. anmms almost had no effect on the weed growth both in height, fresh and dry H. annuus- P. polystachyon and /. batatas- P.polystachyon grown together in a pot (0-5; 1-4; 2-3; 3-2; 4-1; and 5-0) for 5 weeks; 4 replications each, gave another result; both had no effect on height and fresh weight of P. polystachyon. H. annuus­ P. po/ystachyon and I. halalas- P. polystachyon 4-1 reduced the dry weight significantly up to 33.44 and 39.63 %."

"Telah dilakukan penelitian mengenai pola pewarisan warna bunga Zinnia elegans pada kelompok white dan red purple. Penelitian bertujuan untuk mengetahui warna bunga Z. elegans yang muncul dari parental berwarna putih (white group) dan pink keunguan (red purple group), sekaligus mengetahui pola pewarisan warna bunga Z. elegans yang tumbuh di alam. Penelitian dilakukan selama ±6 bulan dari bulan Januari sampai Juni 2020 menggunakan lima bunga, terdiri atas 2 set dari kelompok white (F0) dan 2set dari kelompok red purple (F0), dan 1 set dari kelompok red purple (F1) yang dijadikan sebagai parental. Hasil penelitian menunjukkan bahwa sifat warna bunga dari masing-masing parental diturunkan pada keturunannya. Warna baru dihasilkan dari set 1 kelompok white red purple. Warna bunga pada Zinnia elegans dapat dikelompokkan menjadi kelompok red, red purple, orange, yellow, dan white. Berdasarkan daftar warna (colour chart), masing-masing kelompok warna bunga memiliki intensitas warna yang beragam. Warna bunga yang dihasilkan dari kelompok pink keunguan lebih beragam daripada kelompok putih. Berdasarkan perhitungan chi-square, sebagian besar pola pewarisan Z. elegans yang dibiarkan tumbuh di alam mengikuti pola pewarisan dengan Z. elegans yang disilangkan oleh manusia, dan memiliki pola pewarisan yang berbeda dengan Z. elegans yang telah dimutasi.

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Research on the inheritance pattern of Zinnia elegans from white and red-purple groups has been carried out. This research studies the color of Z. elegans flowers produced from white and red-purple flowers, while knowing the inheritance pattern of Z. elegans flowers in nature. This research was conducted for about six months from January to June 2020 and used four flowers, consisting of 2 sets of white groups (F0), two sets of red-purple groups (F0), and one set of red-purple groups (F1) to be used as parental. The results show the color nature of each parent produced in their generation. New colors are produced from set 1 of the white and red-purple group. The color of flowers in Z. elegans can be grouped into red, red-purple, orange, yellow, and white. Based on the color chart, each flower color group has a variety of color intensities. The color of the flowers produced from the red-purple group is more diverse than the white group. Based on the Chi-square calculation, most of the inheritance patterns of Z. elegans that are allowed to grow in nature follow the pattern resulting from crossing by humans and have different inheritance patterns from Z. elegansobtained from mutation."

"Penelitian jumlah kromosom Asteraceae di lingkungan Kampus Universitas Indonesia (UI) Depok telah dilakukan sebelumnya pada tahun 2013. Dilaporkan bahwa jumlah kromosom 8 dari 21 spesies Asteraceae yang ada di lingkungan tersebut telah berhasil dihitung, dan 5 di antaranya memiliki variasi jumlah kromosom. Penelitian ini dilakukan untuk melengkapi data jumlah kromosom Asteraceae yang ada di lingkungan Kampus UI Depok. Telah dilakukan penghitungan jumlah kromosom ujung akar Porophyllum ruderale, Youngia japonica, Cosmos caudatus, Synedrella nodiflora, Ageratum conyzoides, Cyanthillium cinereum, dan Chromolaena odorata pada bulan April hingga Juni 2015. Jumlah kromosom 5 spesies Asteraceae yang berhasil ditentukan adalah Cosmos caudatus (2n=ca.22, 2n=ca.26, 2n=ca.32, 2n=ca.36, 2n=ca.38, 2n=ca.40, dan 2n=ca.44), Synedrella nodiflora (2n=ca.18, 2n=ca.26, 2n=ca.29, 2n=ca.34, 2n=ca.36, 2n=37, 2n=39, dan 2n=40), Ageratum conyzoides (2n=37 dan 2n=ca.42), Cyanthillium cinereum (2n=9, 2n=16, dan 2n=18), dan Chromolaena odorata (2n=ca.40, 2n=ca.44, 2n=57, dan 2n=60). Cosmos caudatus, Synedrella nodiflora, Cyanthillium cinereum, dan Chromolaena odorata bersifat mixoploid. Mixoploidi tidak dapat ditentukan pada spesies Ageratum conyzoides.

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Study of chromosome number of Asteraceae at Universitas Indonesia (UI) Campus Depok has been conducted previously in 2013. Result has been reported on chromosome numbers of 8 from 21 Asteraceae species at Universitas Indonesia, and 5 of them have variation in chromosome number. This study was addressed to complete chromosome number data of Asteraceae at Universitas Indonesia Campus Depok. Root tips chromosome counting of Porophyllum ruderale, Youngia japonica, Cosmos caudatus, Synedrella nodiflora, Ageratum conyzoides, Cyanthillium cinereum, dan Chromolaena odorata has been done from April to June 2015. Result shows that 5 species chromosome numbers are Cosmos caudatus (2n=ca.22, 2n=ca.26, 2n=ca.32, 2n=ca.36, 2n=ca.38, 2n=ca.40, and 2n=ca.44), Synedrella nodiflora (2n=ca.18, 2n=ca.26, 2n=ca.29, 2n=ca.34, 2n=ca.36, 2n=37, 2n=39, and 2n=40), Ageratum conyzoides (2n=37 and 2n=ca.42), Cyanthillium cinereum (2n=9, 2n=16, and 2n=18), and Chromolaena odorata (2n=ca.40, 2n=ca.44, 2n=57, and 2n=60). Cosmos caudatus, Synedrella nodiflora, Cyanthillium cinereum, and Chromolaena odorata are mixoploid. Mixoploidy cannot be determined on Ageratum conyzoides."

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Penelitian yang bertujuan untuk menganalisis pola pewarisan bentuk bunga Zinnia elegans yang tumbuh dari biji bunga tunggal (single) dan ganda (double) telah dilakukan pada Januari 2020 – Juni 2020. Penelitian dilakukan di 3 lokasi, yaitu Universitas Indonesia, Kelurahan Tanah Baru Depok, dan Kelurahan Grogol Depok. Analisis dilakukan pada 2 generasi tanaman, yaitu pada 237 bunga generasi 1 dan 13 bunga generasi 2. Pengamatan yang dilakukan memerhatikan pola pewarisan jumlah lapisan bunga dan tipe helaian bunga di alam. Hasil yang didapat akan dibandingkan dengan pola pewarisan hukum Mendel, apakah sejalan atau tidak. Berdasarkan hasil penelitian, ditemukan dugaan bahwa lapisan bunga tunggal merupakan tipe yang stabil dan dapat mendominasi tanaman secara alami. Penelitian yang dilakukan juga menduga adanya variasi tipe helaian bunga yang muncul akibat kondisi lingkungan. Tanaman yang berasal dari parental bunga tunggal menunjukkan adanya variasi pola pewarisan, yaitu dapat mengikuti hukum mendel ataupun tidak. Pola pewarisan tanaman yang berasal dari parental bunga ganda belum dapat digambarkan akibat minimnya jumlah tanaman. Pewarisan tipe helaian bunga belum dapat dihubungkan dengan pewarisan Mendel karena informasi tentang tipe helaian bunga sangat terbatas. Pola pewarisan bentuk bunga dari penelitian ini digambarkan dalam bentuk pohon keluarga (pedigree).

 

 

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Research to analyze the inheritance pattern on Zinnia elegans flower shape was conducted in January 2020 – June 2020. The research was conducted in 3 locations, namely Universitas Indonesia Campus, Kelurahan Tanah Baru Depok, and Kelurahan Grogol Depok. The analysis was conducted on 2 generations of plants, on 237 flowers from first generation and 14 flowers from second generation. The flower shape in nature that has been observed were the number of flower layer and the flower sheet. The results in this research will be compared to Mendelian inheritance pattern, whether follow the rules or not. Based on the results of the study, it was found that a single layer of flower was stable and can dominate the plant naturally. This research also assume that the variation type of flower sheet happen due to environmental conditions. Plants that come from a single layer flower show variation in inheritance patterns, which can either follow the law of Mendel or not. The pattern of the plants derived from double layer flower cannot be describe due to lack of plant quantities. The pattern of flower sheet type cannot be linked to Mendelian inheritance because there is only limited number of studies that provide the information. Inheritance pattern of Zinnia elegans’s flower form has been shown on the family tree (pedigree).

 

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"Telah dilakukan penelitian untuk mengetahui bahwa ekstrak etanol rimpang temu mangga Curcuma mangga Val. berpengaruh terhadap kadar bilirubin total dan bilirubin direct akibat kerusakan hati yang diinduksi oleh karbon tetraklorida CCl4 . Hewan uji yang digunakan dalam penelitian yaitu 24 ekor tikus Rattus norvegicus L. jantan galur Sprague-Dawley yang dibagi menjadi enam perlakuan KK1, KK2, KP1, KP2, KP3, dan KP4 dengan empat kali ulangan. Kelompok KK1 merupakan kelompok kontrol normal yang tidak diinjeksikan CCl4 dan dicekok CMC 0.5 , KK2 merupakan kelompok kontrol perlakuan yang diinjeksikan CCl4 sebanyak 1 ml/kgBB secara intraperitoneal dan dicekok CMC 0,5 . Kelompok KP1, KP2, KP3, dan KP4 merupakan kelompok perlakuan yang diinjeksikan CCl4 1 ml/kgBB dan diberikan ekstrak temu mangga dengan dosis berturut-turut 10 mg/kgBB, 20 mg/kgBB, 40 mg/kgBB, dan 80 mg/kgBB. Hasil uji non parametrik Kruskal-Wallis ? = 0,05 menunjukkan bahwa dosis 10 mg/kgBB, 20 mg/kgBB, 40 mg/kgBB, dan 80 mg/kgBB berpengaruh terhadap kadar bilirubin total dan bilirubin direct. Hasil uji perbandingan berganda Dunnett T3 ? = 0,05 menunjukkan bahwa dosis-dosis tersebut tidak berbeda bermakna dengan KK1. Dengan demikian dosis-dosis tersebut memiliki efek kuratif karena dapat menurunkan kadar bilirubin total dan direct sampai mendekati kadar normal.

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This study was conducted in order to observe that the ethanol extract of mango ginger rhizome Curcuma mangga Val. affect the level of total bilirubin and direct bilirubin due to liver damage induced by tetrachloride carbon CCl4 . The test animals in the study were 24 male rats Rattus norvegicus L. of Sprague Dawley strain that was divided into six treatment KK1, KK2, KP1, KP2, KP3 and KP4 and repeated four times. The KK1 group is a normal control group that was not injected with CCl4 and 0.5 CMC fed, KK2 group is a treatment group that was intraperitoneally injected with CCl4 treatment in the amount of 1 ml kgBW and 0.5 CMC fed. KP1, KP2, KP3 and KP4 are treatment groups that got injected with CCl4 1 ml kgBB and were given mango ginger rhizome ethanol extract each with a dose of 10 mg kgBW, 20 mg kgBW, 40 mg kgBW, and 80 mg kgBW respectively by oral. The results of Kruskal Wallis non parametric test 0,05 shows that the dose of 10 mg kgBW, 20 mg kgBW, 40 mg kgBW, and 80 mg kgBW impacted on total bilirubin and direct bilirubin levels. Dunnet rsquo s T3 0,05 multiple comparison test result shows that the dosages had no significant differences with KK1 group. In conclusions, the dosages could be deemed as have curative effects since they successfully reduce the level of total bilirubin and direct bilirubin until it approached normal level."

"Penelitian untuk mengetahui pengaruh jenis pretreatment terhadap kromosom Hibiscus rosa-sinensis telah dilakukan sejak Agustus 2016 hingga Mei 2017. Penelitian menggunakan dua faktor yaitu faktor pretreatment air dingin, paradichlorobenzene PDB, hydroxyquinoline OQ dan PDB:OQ 1:1 dengan variasi lama perendaman 3 jam, 6 jam, 12 jam dan 24 jam. Pengaruh masing-masing pretreatment terhadap fase pembelahan sel dari pucuk batang dapat dilihat melalui persentase interfase, profase awal, profase akhir, metafase, anafase, dan telofase. Morfologi kromosom dan jumlah kromosom juga diamati. Jumlah profase awal dan profase akhir yang tinggi, serta jumlah interfase, metafase, anafase, dan telofase yang rendah digunakan untuk penentuan Pretreatment yang bekerja optimal. Hasil penelitian menunjukkan pretreatment air dingin dengan lama perendaman 3 jam merupakan pretreatment terbaik untuk observasi kromosom. Morfologi kromosom Hibiscus rosa-sinensis L. yang diperoleh berukuran kecil, dengan jumlah kromosom banyak 2n=ca 28 mdash;67 dan bersifat miksoploidi.

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Study to know the effect of pretreatment to Hibiscus rosa sinensis L. chromosome has been carried on since August 2016 to May 2017. There was 2 factors that used, the pretreatment factors cold water, paradichlorobenzene PDB, hydroxyquinoline OQ, and PDB combined with OQ 1 1 and the soaking time length factors 3 hours, 6 hours, 12 hours and 24 hours. The influence of each pretreatment to the phase of cell division of shoot tip could be seen through the percentage of interphase, early prophase, late prophase, metaphase, anaphase, and telophase.

Chromosome morphology and chromosome number also could be observed. The high number of early and late prophase, as well as low number of interphase, metaphase, anaphase, and telophase, indicate that the pretreatment was optimum.

The results showed that pretreatment with cold water in 3 hours was optimum condition for chromosome obervation. The chromosome of Hibiscus rosa sinensis L obtained in this study has small size with large amount in number 2n ca 28 mdash 67 and mixoploid."

"Penelitian yang bertujuan untuk mengetahui pengaruh logam berat timbal (Pb) terhadap perkecambahan biji kangkung (Ipomoea aquatica Forsk.) telah dilakukan. Konsentrasi timbal (Pb) yang digunakan yaitu 0, 50, 100, 150, dan 200 μM. Beberapa parameter telah diamati pada uji perkecambahan. Uji perkecambahan dilakukan dengan mengecambahkan masing-masing lima belas biji kangkung dengan enam ulangan dengan media perkecambahan berupa rockwool. Data yang diperoleh kemudian dianalisis menggunakan one-way ANOVA. Hasil uji perkecambahan menunjukkan terjadinya penurunan nilai seiring bertambahnya konsentrasi timbal (Pb) terhadap parameter berat basah tajuk dan akar, panjang tajuk dan akar, indeks vigor bibit/seedling vigor index (SVI), dan indeks toleransi (TI).

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The research study aimed to determine the effect of heavy metal lead (Pb) on the germination of water spinach (Ipomoea aquatica Forsk.). Lead (Pb) concentrations of 0, 50, 100, 150, and 200 μM were used. Several parameters were observed during the germination test, which involved germinating fifteen water spinach seeds per rockwool support with six replicates. The obtained data were analyzed using one-way ANOVA. The results of the germination test showed a decrease in shoot and root fresh weight, shoot and root length, seed vigor index (SVI), and tolerance index (TI ) as the lead (Pb) concentration increased."

"ABSTRAKEfficiency of water usage is needed for sustainable agriculture. The aim of this research is to investigate the hydroponic (NFT, DFT, Aeroponic) efficiency on water use using Amaranthus viridis L. as the planted material. Efficiency on water use was measured by comparing the water use on hydroponic with the control during the plant growing period. The result shows that the three type of hydroponics are able to increase efficiency of water use. NFT is the most efficient technique with efficiency on water use 20.43%. While DFTand Aeroponic, has the efficiency on water use 12.29% and 3.57%, respectively. Enhancing efficiency of water use on hydroponic was caused by the clossing and circulating irrigation technique on hydroponic are able to minimize the evaporation . "

"ABSTRAKSweet sorghum is a kind of sorghum that contains high content of sugar in its stem. Sweet sorghum has a big potential to be developed in Indonesia owing to its wide adaptation and the fact that it can be used as raw material for liquid sugar, syrup, ethanol, and also as animal feed. Sweet sorghum has not been developed in Indonesia because of lack of a sweet sorghum variety. Improvement of available sweet sorghum genotype can be done among others through plant breeding program. First step on the plant breeding program is to increase the plant genetic variability. This might be done by introduction of varieties or by breeding to create new varieties. Induced mutation using Gamma irradiation can be used to increase the genetic variability of sweet sorghum. Mutation breeding using Gamma irradiation in sweet sorghum was aimed at improving the yield and quality of sweet sorghum. This research was conducted to study the effect of Gamma irradiation on sweet sorghum growth in the M1 generation, and to estimate the optimal dose range suitably for the breeding program. Beside, the objective of this research was to evaluate the genetic variability for the purpose of plant selection in the M2 generation. Plant materials consisted of 2 sweet sorghum lines introduced from ICRISAT namely line No. 79 and No. 83. Non-saccharin sorghum of local variety Higari was used as a control. The doses of Gamma irradiation treatment were 0, 100, 200, 300, 400, 500, 600, 700, 800, 900, and 1000 Gy. The M1 plants were sown in greenhouse at PATIR-BATAN Jakarta, and then were transplanted in the experimental field at Balitbiogen, Bogor. The M2 plants were grown in the experimental field at Lubang Buaya, Jakarta. Important agronomic traits such as plant height, spike length, stem diameter, and grain weight/spike were observed. The results indicated that sorghum lines gave different response to Gamma irradiation, and all measured variables were significantly affected. Irradiation gave morphology and physiology damages on sorghum like abnormality, sterility, and lethality in the M1 generation. The increase of irradiation doses increased physiological damage. Effective doses of Gamma irradiation for sweet sorghum was to be around 400?500 Gy, and the lethal doses 50% of sweet sorghum was around 800?1000 Gy. Putative mutation sometimes could be observed in the M2 generation. The treatment of Gamma increased genetic variability of plant height, spike length, stem diameter, and grain weight/spike. The highest genetic variability was found in the dose treatment of 200?300 Gy. Within this interval dose, there might be high probability to find desirable mutants for further breeding purpose. A number of 38 plants had been selected from the M2 population as putative mutants.

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