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"Bekatul merupakan salah satu produk sampingan sebanyak 10% dari seluruh hasil penggilingan padi. Bekatul mengandung sekitar 12-18,5% minyak bekatul yang menjadi sumber senyawa-senyawa bioaktif yang bermanfaat. Kandungan-kandungan bioaktif dan asam lemak di dalam minyak bekatul dapat dimanfaatkan pada bidang kesehatan. Peningkatan hasil rendemen minyak bekatul dapat dilakukan dengan melakukan proses fermentasi padat menggunakan kapang Aspergillus terreus. Faktor yang dapat mempengaruhi proses fermentasi bekatul diantaranya adalah rasio massa penambahan sumber nutrisi dan jenis sumber nitrogen tambahan. Penelitian ini memvariasikan rasio massa penambahan karbon dan nitrogen sebagai sumber nutrisi sebesar 25:1, 30:1, 35:1, 40:1, dan 45:1. Kedua faktor tersebut dapat mempengaruhi hasil metabolisme kapang dalam peningkatan asam lemak. Bekatul yang telah difermentasi, akan diekstraksi menggunakan metode sonikasi secara bertingkat dengan pelarut n-heksana dan etanol. Analisis kandungan yang terdapat di dalam minyak bekatul akan dilakukan dengan menggunakan instrumen Liquid Chromatography-Mass Spectrometry (LC-MS). Hasil penelitian ini menunjukkan bahwa jenis nitrogen tambahan yang cocok untuk sumber nutrisi Aspergillus terreus pada fermentasi padat dengan medium bekatul adalah jenis nitrogen organik (pepton) dengan rendemen minyak bekatul sebesar 10,46% dan rasio massa penambahan sumber nutrisi karbon dan nitrogen adalah rasio 45:1 dengan rendemen minyak bekatul sebesar 10,40%. Proses ekstraksi dari sampel hasil fermentasi padat bekatul dengan kapang Aspergillus terreus untuk menghasilkan minyak bekatul terbanyak adalah dengan menggunakan pelarut polar (etanol). Senyawa antioksidan yang teridentifikasi pada ekstrak minyak bekatul yaitu asam linoleat dan asam a-linolenat

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Rice bran is one of the by-products, accounting for about 10% of the total rice milling yield. Rice bran contains approximately 12-18.5% rice bran oil, which serves as a source of beneficial bioactive compounds. The bioactive contents and fatty acids in rice bran oil is beneficial for health. To increase the rice bran oil yield, solid-state fermentation processes can be conducted using Aspergillus terreus. Factors affecting the rice bran fermentation process include the mass ratio of nutrient sources addition in fermentation medium and the addition of nitrogen sources. This study varied the mass ratio of carbon and nitrogen addition as a nutrient source in fermentation medium at 25:1, 30:1, 35:1, 40:1, and 45:1. Both factors can affect fungal metabolism and the increase in fatty acids during rice bran fermentation. After that, the result of rice bran fermentation process will undergo an extraction process using a multi-step sonication method with n-hexane and ethanol as solvents. The content of rice bran oil will be analyze using Liquid Chromatography-Mass Spectrometry (LC-MS). The results of this study indicate that the suitable type of additional nitrogen for Aspergillus terreus nutrition in solid-state fermentation with rice bran medium is organic nitrogen (peptone), yielding rice bran oil at 10,46%. The optimal mass ratio of added carbon and nitrogen nutrients is 45:1, resulting in a rice bran oil yield of 10.40%. The extraction process of rice bran oil from the solid-state fermentation sample with Aspergillus terreus is most effective using a polar solvent (ethanol). The identified antioxidant compounds in the rice bran oil extract include linoleic acid and a-linolenic acid."

"Semakin berkembangnya zaman, membuat pembangunan infrastruktur harus dapat dilakukan di berbagai tempat, begitu pun pada tanah gambut. Akan tetapi, tanah gambut merupakan salah satu jenis tanah yang memiliki kekuatan yang buruk dan kurang baik sebagai dasar konstruksi sipil. Oleh karena itu, diperlukan sebuah usaha untuk stabilisasi atau meningkatkan daya dukung tanah gambut, yaitu pada penelitian ini akan dilakukan penambahan mikroorganisme selulolitik pada tanah gambut yang bertujuan untuk menguraikan atau mendekomposisi senyawa organik berupa serat dan selulosa menjadi senyawa anorganik yang lebih sederhana dan padat. Volume mikroorganisme yang ditambahkan adalah sebesar 10% dari volume tanah dalam wadah per tahap dari total 2 tahap injeksi dan masa fermentasi selama 65 hari. Pengujian kekuatan tanah yang dilakukan adalah uji triaksial Consolidated Undrained (CU) dan Unconsolidated Undrained (UU). Setelah dilakukan injeksi mikroorganisme dan fermentasi, didapatkan hasil peningkatan parameter kuat geser tanah gambut yaitu, nilai kohesi dan sudut geser.

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As the time goes by, constructing infrastructure must be able to do in various places, even on the peat soil area. However, peat soil is a type of soil which have a low strength and not good enough as a base of civil construction. According to that condition, it takes an effort to stabilize or increase the bearing capacity of peat soil, this study will be use the addition of cellulolytic microorganisms in peat soil that aims to decompose the fiber and cellulose into solid and simpler organic compounds. Volume of microorganisms that are added is equal to 10% of the total volume of soil in the container per stage of total 2 stages and with fermentation period for 65 days. The soil strength test which was conducted in this experiment is Consolidated Undrained (CU) and Unconsolidated Undrained (UU) triaxial tests. After the injection of microorganisms and 65 days fermentation, the result showed an increasing in shear strength of peat soil parameters, the value of cohesion and friction angle."

"[ABSTRAKAspergillus flavus UICC 360 merupakan fungi yang mampu menghasilkan senyawa metabolit sekunder berupa lovastatin. Penelitian bertujuan untuk mengetahui pengaruh variasi konsentrasi urea terhadap kemampuan Aspergillus flavus UICC 360 dalam menghasilkan lovastatin. Proses fermentasi menggunakan konsentrasi inokulum Aspergillus flavus UICC 360 sebesar 1,96% (v/v) dalam medium Czapek’s Dox Broth (CDB) modifikasi dengan variasi konsentrasi urea (0 mM, 33 mM, 42 mM, 50 mM, 58 mM, dan 67 mM) dan inkubasi selama 7 hari pada suhu ruang (27--300C) dengan kecepatan agitasi 90 rpm. Ekstrak hasil fermentasi dalam etil asetat diuji terhadap Candida albicans UICC Y-29 menggunakan metode difusi agar cara cakram. Ekstrak hasil fermentasi dari konsentrasi urea 42 mM mempunyai indeks penghambatan rata-rata tertinggi sebesar 0,54 ± 0,15. Hasil Kromatografi Lapis Tipis (KLT) menunjukkan bahwa nilai Rf ekstrak hasil fermentasi dari konsentrasi urea 42 mM sama dengan lovastatin standar, yaitu 0,42 yang mengindikasikan ekstrak mengandung lovastatin. Uji Least Significant Difference (LSD) (P < 0,05) menunjukkan terdapat perbedaan nyata variasi konsentrasi urea terhadap kemampuan Aspergillus flavus UICC 360 dalam menghasilkan lovastatin. Hal tersebut menunjukkan bahwa pemberian variasi konsentrasi urea berpengaruh terhadap kemampuan Aspergillus flavus UICC 360 dalam menghasilkan lovastatin.

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ABSTRACTAspergillus flavus UICC 360 is capable of producing secondary metabolites such as lovastatin. The study aims to determine the effect of variations of urea concentration on the ability of Aspergillus flavus UICC 360 to produce lovastatin. The fermentation process using 1.96% (v/v) inoculum concentration of Aspergillus flavus UICC 360 in the Czapek’s Dox Broth (CDB) medium modified with urea concentration variations (0 mM, 33 mM, 42 mM, 50 mM, 58 mM, and 67 mM) and incubated for 7 days at room temperature (27--30 °C) with agitation speed of 90 rpm. Ethyl acetate extracts were tested against Candida albicans UICC Y-29 using agar disc diffusion method. The extract from fermentation medium of 42 mM urea has the highest average of inhibition index of 0.54 ± 0.15. Results of Thin Layer Chromatography (TLC) showed that the extract from fermentation medium of 42 mM urea has the same Rf value with lovastatin standard Rf 0.42 which indicated that the extract contained lovastatin. Least Significant Difference (LSD) test showed that there were significant differences in the urea concentration variation in the ability of Aspergillus flavus UICC 360 to produce lovastatin. It shows that variation of urea concentrations affect the ability of Aspergillus flavus UICC 360 to produce lovastatin.;Aspergillus flavus UICC 360 is capable of producing secondary metabolites such as lovastatin. The study aims to determine the effect of variations of urea concentration on the ability of Aspergillus flavus UICC 360 to produce lovastatin. The fermentation process using 1.96% (v/v) inoculum concentration of Aspergillus flavus UICC 360 in the Czapek’s Dox Broth (CDB) medium modified with urea concentration variations (0 mM, 33 mM, 42 mM, 50 mM, 58 mM, and 67 mM) and incubated for 7 days at room temperature (27--30 °C) with agitation speed of 90 rpm. Ethyl acetate extracts were tested against Candida albicans UICC Y-29 using agar disc diffusion method. The extract from fermentation medium of 42 mM urea has the highest average of inhibition index of 0.54 ± 0.15. Results of Thin Layer Chromatography (TLC) showed that the extract from fermentation medium of 42 mM urea has the same Rf value with lovastatin standard Rf 0.42 which indicated that the extract contained lovastatin. Least Significant Difference (LSD) test showed that there were significant differences in the urea concentration variation in the ability of Aspergillus flavus UICC 360 to produce lovastatin. It shows that variation of urea concentrations affect the ability of Aspergillus flavus UICC 360 to produce lovastatin.;Aspergillus flavus UICC 360 is capable of producing secondary metabolites such as lovastatin. The study aims to determine the effect of variations of urea concentration on the ability of Aspergillus flavus UICC 360 to produce lovastatin. The fermentation process using 1.96% (v/v) inoculum concentration of Aspergillus flavus UICC 360 in the Czapek’s Dox Broth (CDB) medium modified with urea concentration variations (0 mM, 33 mM, 42 mM, 50 mM, 58 mM, and 67 mM) and incubated for 7 days at room temperature (27--30 °C) with agitation speed of 90 rpm. Ethyl acetate extracts were tested against Candida albicans UICC Y-29 using agar disc diffusion method. The extract from fermentation medium of 42 mM urea has the highest average of inhibition index of 0.54 ± 0.15. Results of Thin Layer Chromatography (TLC) showed that the extract from fermentation medium of 42 mM urea has the same Rf value with lovastatin standard Rf 0.42 which indicated that the extract contained lovastatin. Least Significant Difference (LSD) test showed that there were significant differences in the urea concentration variation in the ability of Aspergillus flavus UICC 360 to produce lovastatin. It shows that variation of urea concentrations affect the ability of Aspergillus flavus UICC 360 to produce lovastatin., Aspergillus flavus UICC 360 is capable of producing secondary metabolites such as lovastatin. The study aims to determine the effect of variations of urea concentration on the ability of Aspergillus flavus UICC 360 to produce lovastatin. The fermentation process using 1.96% (v/v) inoculum concentration of Aspergillus flavus UICC 360 in the Czapek’s Dox Broth (CDB) medium modified with urea concentration variations (0 mM, 33 mM, 42 mM, 50 mM, 58 mM, and 67 mM) and incubated for 7 days at room temperature (27--30 °C) with agitation speed of 90 rpm. Ethyl acetate extracts were tested against Candida albicans UICC Y-29 using agar disc diffusion method. The extract from fermentation medium of 42 mM urea has the highest average of inhibition index of 0.54 ± 0.15. Results of Thin Layer Chromatography (TLC) showed that the extract from fermentation medium of 42 mM urea has the same Rf value with lovastatin standard Rf 0.42 which indicated that the extract contained lovastatin. Least Significant Difference (LSD) test showed that there were significant differences in the urea concentration variation in the ability of Aspergillus flavus UICC 360 to produce lovastatin. It shows that variation of urea concentrations affect the ability of Aspergillus flavus UICC 360 to produce lovastatin.]"

"Perkembangan teknologi dan industri mendorong meningkatnya permintaan akan lipase sebagai biokatalis namun secara komersial ketersediaan enzim lipase masih terbatas dan harga jualnya pun mahal. Kebutuhan ini dapat diatasi dengan produksi lipase ekstraseluler dari mikroba dengan fermentasi substrat padat (SSF). Pada penelitian ini dilakukan sintesis enzim lipase ekstraseluler dari kapang Aspergillus niger dengan menggunakan metode SSF pada substrat fermentasi berupa residu agroindustri seperti ampas tahu, ampas kelapa, dan dedak jagung dan diamati juga pengaruh kondisi fermentasi seperti konsentrasi induser serta waktu fermentasi terhadap aktivitas lipase yang dihasilkan. Jenis substrat yang menghasilkan aktivitas supernatant lipase terbaik adalah ampas tahu dengan konsentrasi induser 4% selama 9 hari fermentasi, dengan unit aktivitas 8,48 U/mL. Supernatant lipase ini dikeringkan dengan metode spray drying dan kemudian diimobilisasi pada resin anion macroporous dengan metode adsorpsi-crosslinking. Lipase terimobilisasi diuji aktivitasnya dalam reaksi sintesis biodiesel rute non-alkohol di reaktor batch dengan perbandingan mol reaktan minyak kelapa sawit dan metil asetat sebesar 1:12, pada suhu reaksi 40oC selama 50 jam, dan menghasilkan 48.3% yield biodiesel. Stabilitas aktivitas enzim juga diuji dengan penggunaan pada 4 siklus reaksi sintesis biodiesel, dan enzim masih memiliki 84% dari aktivitas awal meskipun telah digunakan dalam 4 siklus reaksi.

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The advancement of technology pushes the rise of demand for lipase as a biocatalyst but commercially, the availability of lipase is still very limited and the price is very expensive. To fill in this needs, extracellular lipase enzyme from Aspergillus niger can be produced by solid state fermentation (SSF) using agroindustrial wastes including tofu dregs, coconut dregs, and corn bran. The aforementioned agroindustrial residues still contain nutritions, especially lipids/triglycerides which makes them potential as fermentation medium to produce lipase. Lipase with the highest activity is obtained from tofu dregs as substrate with 4% inducer in 9 days fermentation, which shows activity of 8.48 U/mL.

This crude lipase extract is then dried with spray dryer and then immobilized in anion macroporous resin with adsorption-crosslinking as the immobilization method. The immobilized lipase?s activity is then assayed by biodiesel synthesis reaction and shows 48.3% yield. The immobilized enzyme's stability is also tested with four cycles of biodiesel synthesis, and in the fourth cycle, it still maintained 84% of its initial activity."

"Aspergillus flavus UICC 360 has been reported to produce lovastatin. This research was carried out to determine the effect of concentration variation of glucose technical grade on the ability of A. flavus UICC 360 to produce lovastatin. The fermentation process was carried out using inoculum 2% (v/v) modified Czapek's Dox Broth (CDB). Variation of glucose technical grade concentration used were 0 g/L, 5 g/L, 10 g/L, 15 g/L, 20 g/L, 25 g/L, 30 g/L and 35 g/L. Fermentation was carried out for 6 days at room temperature (27--30ºC) with agitation speed of 90 rpm. Extraction of lovastatin was done with ethyl acetate solvent. The extract was assayed by disk diffusion method against Candida albicans UICC Y-29.

The results revealed that the fermentation extract on glucose technical grade at 15 g/L showed the highest inhibition index of 0.77 ± 0.09. Analysis using Least Significant Difference (LSD) (P < 0.05) showed there was significant difference on the ability of A. flavus UICC 360 to produce lovastatin at different glucose technical grade concentration. High Performance of Liquid Chromatography (HPLC) showed that concentration of 15 g/L glucose technical grade had the same retention time with standard lovastatin at 4.52 minutes and 54.2 mg/L concentration."

"Commercialization ofbioethanol has recently intensified due to its market stability, low cost,sustainability, alternative fuel energy composition, greener output andcolossal fossil fuel depletion. Recently, because of greenhouse intensityworldwide, many researches are ongoing to reprocess the waste as well asturning down the environmental pollution. With this scenario, the invention ofbioethanol was hailed as a great accomplishment to transform waste biomass tofuel energy and in turn reduce the massive usages of fossil fuels. In thisstudy, our review enlightens various sources of plant-based waste feed stocksas the raw materials for bioethanol production because they do not adverselyimpact the human food chain. However, the cheapest and conventionalfermentation method, yeast fermentation is also emphasized here notably forwaste biomass-to-bioethanol conversion. Since the key fermenting agent, yeastis readily available in local and international markets, it is morecost-effective in comparison with other fermentation agents. Furthermore, yeasthas genuine natural fermentation capability biologically and it produces zerochemical waste. This review also concerns a detailed overview of the biologicalconversion processes of lignocellulosic waste biomass-to-bioethanol, thediverse performance of different types of yeasts and yeast strains,plusbioreactor design, growth kinetics of yeast fermentation, environmentalissues, integrated usages on modern engines and motor vehicles, as well asfuture process development planning with some novel co-products."

"Penelitian ini bertujuan menguji kemampuan tiga strain R. delemar UICC 27, UICC 67 dan UICC 121 dalam memfermentasi campuran lumpur dan bungkil sawit 3:1 dan 4:1 nonsteril serta 3:1 dan 4:1 steril, selanjutnya menganalisis perubahan komposisi karbohidrat, protein, lemak, dan abu campuran limbah sebelum dan setelah fermentasi. Data pendukung meliputi pengamatan morfologi, penghitungan jumlah sel/mL, kepadatan miselium, sporulasi; serta pengamatan campuran lumpur dan bungkil sawit meliputi warna, aroma, pH dan kekompakan. Kemampuan fermentasi ditandai dengan pertumbuhan ketiga strain R. delemar pada campuran limbah. Rhizopus delemar UICC 27, UICC 67 dan UICC 121, menunjukkan tidak ada pertumbuhan pada campuran lumpur:bungkil sawit 3:1 dan 4:1 nonsteril. Rhizopus delemar UICC 27, UICC 67 dan UICC 121, menunjukkan ada pertumbuhan pada campuran lumpur:bungkil sawit 3:1 dan 4:1 steril. Konsentrasi inokulum sebesar 10 v/b , dengan jumlah sel awal sebanyak 1x107 CFU/mL digunakan dalam campuran dengan berat total 20 g. Hasil enumerasi menunjukkan bahwa terjadi pertumbuhan tiga strain R. delemar pada campuran lumpur dan bungkil 3:1 steril, dan ditunjukkan dengan peningkatan jumlah sel. Pertumbuhan tercepat dan peningkatan jumlah sel terbanyak ditunjukkan oleh R. delemar UICC 67 menjadi 5,5x108CFU/mL, selanjutnya strain UICC 121 menjadi 3,8x108CFU/mL dan UICC 27 menjadi 3,8x108CFU/mL. UICC 67 dipilih sebagai strain potensial untuk memfermentasi campuran limbah dan bungkil sawit 3:1 nonsteril dan steril. Hasil analisis menunjukkan R. delemar UICC 67 mampu mengubah komposisi campuran limbah setelah lima hari fermentasi. Rhizopus delemar strain UICC 67 mampu meningkatkan kandungan karbohidrat dan protein pada campuran limbah steril sebanyak 21,70 dan 30,76 secara berturut-turut, serta menurunkan kandungan lemak total dan kadar abu sebanyak 46,11 dan 15,26 secara berturut-turut.

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This study was to test the ability of the three strains of R. delemar UICC 27, UICC 67 and UICC 121 fermenting a mixture of slurry and palm kernel cake 3:1 dan 4:1 nonsterile and 3:1 dan 4:1 sterile, also analyzing the composition carbohydrates, protein, lipid, and ash of mixtures of slurry and palm kernel cake before and after fermentation. Supporting data includes morphological observation, counting the number of cell, the density of mycelia, sporulation and also observation of the substrate before and after fermentation include color, aroma, pH and compactness. Fermentation ability indicated by the growth of the three strains of R. delemar in the waste mixture. Rhizopus delemar UICC 27, UICC 67 and UICC 121 showed no growth in mixture of slurry and palm kernel cake 3:1 and 4:1 nonsterile. Rhizopus delemar UICC 27, UICC 67 and UICC 121 showed growth in mixture of slurry and palm kernel cake 3:1 and 4:1 sterile. Inoculum concentration of 10 v/w of the total weight from the mixtures as much as 20 g with initial cell number 1x107 CFU mL. The results showed, there are growth of the three strains of R. delemar in the waste mixture with the increased number of cells. The fastest growth and the highest number of cells showed by UICC 67 became 5,5x107 CFU mL. Second, by UICC 27 became 3,8x107 CFU mL, and then by UICC 121 became 3,8x107 CFU mL. Strain R. delemar UICC 67 selected as a potential strain to ferment the mixtures of slurry palm kernel cake 3:1 nonsterile and sterile. The results of the analysis of slurry palm kernel cake 3:1 sterile showed increased in carbohydrate and protein content by 21,70 and 30,76 respectively, and decreased lipid and ash content by 46,11 and 15,26 respectively."

"ABSTRAK Penelitian ini bertujuan menguji kemampuan tiga strain R. microsporus UICC500, UICC 531, dan UICC 539 dalam memfermentasi campuran lumpur danbungkil sawit 3:1 dan 4:1 nonsteril, serta 3:1 dan 4:1 steril, selanjutnya menganalisis perubahan komposisi karbohidrat, lemak, protein, air, dan abu campuran limbah setelah fermentasi. Konsentrasi inokulum sebesar 10 v/b ,dengan jumlah sel awal sebanyak 1x107 CFU/mL digunakan dalam campurandengan berat total 20g. Kemampuan R. microsporus memfermentasi campuran lumpur dan bungkil sawit ditunjukkan melalui pertumbuhan R. microsporus padacampuran limbah sawit, yaitu morfologi, jumlah sel/mL, kepadatan miselium, dansporulasi; pengamatan pada campuran limbah sawit, yaitu warna, kekompakan,aroma, dan pH; serta perubahan komposisi campuran limbah sawit. Hasilpengamatan mengindikasikan Rhizopus microsporus UICC 500, UICC 531, danUICC 539 tidak memfermentasi campuran lumpur dan bungkil 4:1 nonsterilyang ditunjukkan dengan tidak ada pertumbuhan ketiga strain tersebut. Rhizopusmicrosporus UICC 539 memfermentasi campuran lumpur dan bungkil sawit 3:1 nonsteril, 3:1 steril, 4:1 steril yang ditunjukkan dengan adanya pertumbuhan.Rhizopus microsporus UICC 500 dan UICC 531 memfermentasi campuran lumpur dan bungkil sawit 3:1 dan 4:1 steril yang ditunjukkan dengan adanyapertumbuhan. Pertumbuhan tercepat dan jumlah sel terbanyak pada campuranlumpur dan bungkil sawit 3:1 steril ditunjukkan oleh R. microsporus UICC 539yaitu 1,77 x108 CFU/mL, selanjutnya R. microsporus UICC 531 yaitu 1,35 x108CFU/mL, dan R. microsporus UICC 500 yaitu 1,3 x 108 CFU/mL. Rhizopusmicrosporus UICC 539 dapat mengubah komposisi campuran lumpur dan bungkil 3:1 steril setelah lima hari fermentasi, yaitu dapat meningkatkan kandunganprotein, karbohidrat, dan abu pada campuran limbah steril sebanyak 16 , 1,71 ,dan 17,5 secara berturut-turut, serta menurunkan kandungan lemak dan airsebanyak 48 dan 0,1.

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ABSTRACT This study aims to test the ability of three strains of R. microsporus UICC 500,531 UICC and UICC 539 to ferment slurry and kernel cake mixtures 3 1 and4 1 non sterile, and 3 1 and 4 1 sterile, then analyzes the composition change carbohydrates, fats, protein, water and ash of waste mixtures after fermentation.Fermentation of slurry and palm kernel cake mixtures with inoculumconcentration of 10 v w of the total weight from the mixtures as much as 20 gwith initial cell number 1x107 CFU mL. Rhizopus microsporus was able toferment slurry and kernel cake mixtures, showed by growth of R. microsporus onwaste mixtures, includes morphology, number of cells mL, density of mycelium,and sporulation the observation of slurry and kernel cake mixtures, includes color,compactness, odor, and pH, and also the change of waste mixtures rsquo s composition.The observation results indicated that R. microsporus UICC 500, UICC 531, andUICC 539 were unable to ferment the slurry and kernel cake 4 1 non sterilemixtures, showed by no growth. Rhizopus microsporus UICC 539 was able toferment the slurry and kernel cake mixtures 3 1 non sterile, 3 1 sterile, 4 1 sterile, indicated by growth on the mixtures. Rhizopus microsporus UICC 500 andUICC 531 was able to ferment slurry and kernel cake mixtures 3 1 and 4 1 sterile, showed by growth on the mixtures. The fastest growth and the highestnumber of cells showed by Rhizopus microsporus UICC 539 with 1.77 x108CFU mL, then Rhizopus microsporus UICC 531 with 1.35 x108 CFU mL, and R.microsporus UICC 500 with 1.3 x108 CFU mL on slurry and kernel cake mixtures 3 1 sterile. Rhizopus microsporus UICC 539 was able to change the compositionof slurry and kernel cake 3 1 sterile mixtures after five days fermentation. Theprotein, carbohydrates, and ash content increased by 16 , 1.71 , and 17.5 ,respectively, whereas fats and water content decreased by 48 and 0.1 ,respectively."

"Tanah gambut adalah material organik yang berasal dari campuran fragmen-fragmen tumbuhan yang telahmembusuk akibat air endapan dan terbentuk dalam tanah basah yang berubah secara kimia akibat pengaruhcuaca, kondisi topografi, sirkulasi oksigen yang kurang bagus ,dan proses dekomposisi oleh bakteri danmikroorganisme lain di dalam tanah yang tidak sempurna. Tanah gambut termasuk tanah yang bermasalah danpersebarannya banyak ditemukan di beberapa daerah yang memiliki sungai dan rawa lebih banyak seperti diSumatera, Kalimantan dan Papua. Luas tanah gambut di Sumatera Selatan terbanyak kedua yakni 1.43 jutahektar dari 7.14 juta hektar lahan gambut di Sumatera. Untuk mengatasi permasalahan pada tanah gambutdilakukan upaya penstabilan dengan mikroorganisme selulolitik. Uji yang dilakukan sama dengan Pandamean 2014 yaitu dengan uji konsolidasi. Dari uji yang dilkukan dilihat pengaruh perubahan metode injeksi danpenambahan waktu fermentasi terhadap parameter dasar serta parameter kompresibilitas sampel. Dari hasil uji,perubahan metode injeksi serta waktu fermentasi tidak terlalu berpengaruh terhadap perubahan nilai parameterdasar, akan tetapi terdapat perubahan yang cukup besar pada nilai Cc 0.34 berbanding 1.96 dan nilai C 0.01 berbanding 0.02.

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Peat soil is an organic material derived from a mixture of plant fragments that have been decomposed due tosediment water and formed in wet soils that change chemically due to weather effects, topographical conditions,poor oxygen circulation, and decomposition by other bacteria and microorganisms in imperfect soil. Peat soils isone of the problematic soils and their distribution is found in areas with more rivers and swamps such asSumatra, Kalimantan and Papua. The peat soil area in South Sumatra is the second largest area in Sumatra 1.43million hectares of 7.14 million hectares of peatland in Sumatra. To solve the problems of the peat soil, theresearcher do a study about a stability efforts with cellulolytic microorganisms. The test performed is the same asPandamean 2014 by consolidation test. From the test we know that the effect of the injection method changeand the addition of fermentation time to the basic and compressibility parameters. From the test result, thechange of injection method and increasing of fermentation time did not significantly affect the value of the basicparameters, but there was a considerable change in the value of Cc 0.34 versus 1.96 and the C value 0.01 versus 0.02. "

"Asam kojat merupakan metabolit sekunder yang dihasilkan melalui fermentasi kapang genus Aspergillus dan Penicillium yang menggunakan karbohidrat sebagai substrat. Penelitian ini bertujuan untuk mendapatkan kondisi fermentasi yang optimal yang dapat menghasilkan asam kojat dengan nilai yield tertinggi dari kultur campuran Aspergillus oryzae dan Aspergillus tamarii. Optimasi sumber karbon dan nitrogen, nilai pH medium, rasio konsentrasi inokulum, dan kondisi aerasi dilakukan secara bertahap. Dari sembilan variasi medium fermentasi, diperoleh sumber karbon dan nitrogen yang optimal yaitu sukrosa dan yeast extract dengan jumlah asam kojat 2,6163 g/l. Optimasi nilai pH medium yang terdiri dari tiga variasi menghasilkan asam kojat terbanyak pada pH 3,5 sebesar 2,6163 g/l. Optimasi rasio konsentrasi inokulum dilakukan dengan tiga variasi rasio dimana rasio 2 : 3 inokulum A. oryzae dan A. tamarii menghasilkan asam kojat terbanyak sebesar 2,8889 g/l. Optimasi kondisi aerasi dilakukan dengan dua variasi volume medium dimana medium dengan volume 100 ml menghasilkan asam kojat dengan jumlah tertinggi yaitu 6,5594 g/l. Efisiensi dari proses fermentasi ditentukan dengan menghitung nilai yield asam kojat dimana didapatkan yield tertinggi 0,1396 gg-1.

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AbstractKojic acid is a secondary metabolite produced by fermentation of Aspergillus and Penicillium mold using carbohydrate as the substrate. This research aims to determine the optimal fermentation conditions with high yield value from a mixture of Aspergillus oryzae and Aspergillus tamarii cultures. Optimization of carbon and nitrogen sources, pH value of medium, inoculum concentration ratio, and aeration were done gradually. From nine fermentation medium, the most optimal carbon and nitrogen source was sucrose and yeast extract which obtained 2,6163 g l of kojic acid.

Optimization of pH value consisting three various pH obtained 2,6163 g l of kojic acid in medium with pH 3,5. Ratio of inoculum concentration were optimized with three different ratio which the 2 3 of Aspergillus oryzae and Aspergillus tamarii became the most optimal ratio with 2,8889 g l of kojic acid. Aeration optimization was done with two various medium volume which medium with 100 ml volume obtained 6,5594 g l as the highest amount of kojic acid. The efficiency of fermentation was determined by calculating the yield value which was 0,1396 gg 1."